​ Théo Le Moigne – PhD defensis

“Structures and interactions of Calvin-Benson-Bassham cycle enzymes”

under the direction of Julien Henri. 

Ph.D defense which will take place on Tuesday October 4th at 2pm in the Bilski-Pasquier amphitheatre of the Campus des Cordeliers,  15-21 rue de l’École-de-médecine in IBPC’s Conference room and in visioconference

Meeting ID : 811 6454 1331
Password : 839728

Photosynthesis is one of the main processes supporting life. It is composed of two phases, the conversion of light energy and water into reductive power and chemical energy, and the Calvin-Benson-Bassham cycle (CBBC) that fixes atmospheric CO2 into organic molecules. The CBBC is catalyzed by eleven enzymes, essential for photosynthesis and conserved in the green lineage of plants and microalgae. CBBC enzymes are regulated by multiple modes, including post-translational modifications and protein-protein interactions. In order to describe the CBBC system at the molecular level, we aimed at determining the tridimensional structure of these enzymes at high resolution. I purified, crystallized, solved and analyzed the crystal structures of the ribose-5-phosphate isomerase (RPI), the fructose bisphosphate aldolase (FBA), the sedoheptulose-1,7-bisphosphatase (SBPase), and the phosphoglycerate kinase (PGK) from the model green microalga Chlamydomonas reinhardtii, and searched for multi-enzymatic complexes formed by CBBC enzymes in vitro.

The defense will take place in front of a jury composed of :

Mirjam Czjzek, Reviewer and Assessor
Nicolas Rouhier, Reviewer and Assessor
Alix Boulouis, Assessor
Hélène Launay, Assessor
Sylvie Nessler, Assessor